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CD58 Polyclonal antibody

CD58 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10878-1-AP

Synonyms

Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD58 Polyclonal antibody (10878-1-AP)

    WB analysis using 10878-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of TF-1 cells using CD58 Polyclonal antibody (10878-1-AP)

    WB analysis of TF-1 using 10878-1-AP

    TF-1 cells were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CD58 Polyclonal antibody (10878-1-AP)

    WB analysis of HeLa using 10878-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lymph tissue using CD58 Polyclonal antibody (10878-1-AP)

    WB analysis of human lymph using 10878-1-AP

    human lymph tissue were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD58 Polyclonal antibody (10878-1-AP)

    IHC staining of human ovary cancer using 10878-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10878-1-AP (CD58 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CD58 Polyclonal antibody (10878-1-AP)

    IHC staining of human skin cancer using 10878-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10878-1-AP (CD58 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CD58 Polyclonal antibody (10878-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 10878-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10878-1-AP (CD58 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inRaji cells, Jurkat cells, TF-1 cells, HeLa cells, human lymph tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10878-1-AP targets CD58 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD58 fusion protein Ag1165
Full Name CD58 molecule
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC005930
Gene Symbol CD58
Gene ID (NCBI) 965
RRIDAB_2260179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD58 a heavily glycosylated protein of 55-70 kDa, is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells. The protein is localized to the plasma membrane.

Protocols

Product Specific Protocols
WB protocol for CD58 antibody 10878-1-APDownload protocol
IHC protocol for CD58 antibody 10878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Heliyon

Clinical significance of immune-related antigen CD58 in gliomas and analysis of its potential core related gene clusters

Authors - Zhi Tian
View Article