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  • Featured Product
  • KD/KO Validated

CD46 Polyclonal antibody

CD46 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12494-1-AP

Synonyms

TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CD46 Polyclonal antibody (12494-1-AP)

    WB analysis using 12494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12494-1-AP (CD46 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using CD46 Polyclonal antibody (12494-1-AP)

    IP experiment of A549 using 12494-1-AP

    IP result of anti-CD46 (IP:12494-1-AP, 4ug; Detection:12494-1-AP 1:1000) with A549 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CD46 Polyclonal antibody (12494-1-AP)

    IHC staining of human ovary tumor using 12494-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CD46 Polyclonal antibody (12494-1-AP)

    IHC staining of human ovary tumor using 12494-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CD46 Polyclonal antibody (12494-1-AP)

    IHC staining of human cervical cancer using 12494-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CD46 Polyclonal antibody (12494-1-AP)

    IHC staining of human cervical cancer using 12494-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD46 Polyclonal antibody (12494-1-AP)

    IHC staining of human colon cancer using 12494-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD46 Polyclonal antibody (12494-1-AP)

    IHC staining of human colon cancer using 12494-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD46 Polyclonal antibody (12494-1-AP)

    IHC staining of human breast cancer using 12494-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD46 Polyclonal antibody (12494-1-AP)

    IHC staining of human breast cancer using 12494-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD46 Polyclonal antibody (12494-1-AP)

    IF Staining of HeLa using 12494-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CD46 antibody (12494-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, MOLT-4 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human colon cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12494-1-AP targets CD46 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD46 fusion protein Ag3131
Full Name CD46 molecule, complement regulatory protein
Calculated Molecular Weight 384 aa, 43 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC030594
Gene Symbol CD46
Gene ID (NCBI) 4179
RRIDAB_2260042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).

Protocols

Product Specific Protocols
WB protocol for CD46 antibody 12494-1-APDownload protocol
IHC protocol for CD46 antibody 12494-1-APDownload protocol
IF protocol for CD46 antibody 12494-1-APDownload protocol
IP protocol for CD46 antibody 12494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, FC

Mol Immunol

Functional characterization of two novel non-synonymous alterations in CD46 and a Q950H change in factor H found in atypical hemolytic uremic syndrome patients.

Authors - Frida C Mohlin
View Article
humanWB

Oncol Rep

Bioinformatic analysis of the membrane cofactor protein CD46 and microRNA expression in hepatocellular carcinoma.

Authors - Zejun Lu
  • KD Validated
View Article
mouseWB

Clin Exp Immunol

Mitigating placental injuries through up-regulating DAF in experimental APS mice:new mechanism of progesterone.

Authors - Yingchun Zhang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Andrea (Verified Customer) (04-12-2022)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa cells