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  • Featured Product
  • KD/KO Validated

CD44 Polyclonal antibody

CD44 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, Neutralization, ELISA

Conjugate

Unconjugated

Cat no : 15675-1-AP

Synonyms

Epican, ECMR-III, ECMR III, CSPG8, CDW44

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CD44 Polyclonal antibody (15675-1-AP)

    WB analysis of HeLa cells using 15675-1-AP

    WB result of CD44 antibody (15675-1-AP, 1:6000) with si-Control and si-CD44 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using CD44 Polyclonal antibody (15675-1-AP)

    WB analysis using 15675-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD44 Polyclonal antibody (15675-1-AP)

    WB analysis using 15675-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CD44 Polyclonal antibody (15675-1-AP)

    IP experiment of HeLa using 15675-1-AP

    IP result of anti-CD44 (IP:15675-1-AP, 4ug; Detection:15675-1-AP 1:2000) with HeLa cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD44 Polyclonal antibody (15675-1-AP)

    IHC staining of human lung cancer using 15675-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD44 Polyclonal antibody (15675-1-AP)

    IHC staining of human lung cancer using 15675-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD44 Polyclonal antibody (15675-1-AP)

    IHC staining of human colon cancer using 15675-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD44 Polyclonal antibody (15675-1-AP)

    IHC staining of human colon cancer using 15675-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using CD44 Polyclonal antibody (15675-1-AP)

    IHC staining of human oesophagus cancer using 15675-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using CD44 Polyclonal antibody (15675-1-AP)

    IHC staining of human oesophagus cancer using 15675-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CD44 Polyclonal antibody (15675-1-AP)

    IF Staining of human colon cancer using 15675-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using 15675-1-AP (CD44 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD44 Polyclonal antibody (15675-1-AP)

    IF Staining of HeLa using 15675-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of UCMSCs cells using CD44 Polyclonal antibody (15675-1-AP)

    IF Staining of UCMSCs using 15675-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed UCMSCs cells using CD44 antibody (15675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using CD44 Polyclonal antibody (15675-1-AP)

    IF Staining of A375 using 15675-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using CD44 antibody (15675-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CD44 Polyclonal antibody (15675-1-AP)

    IF Staining of HUVEC using 15675-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CD44 antibody (15675-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD44 Polyclonal antibody (15675-1-AP)

    IF Staining of HeLa using 15675-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD44 Polyclonal antibody (15675-1-AP)

    IF Staining of HeLa using 15675-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HUVEC cells, MDA-MB-231 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inHeLa cells, human colon cancer tissue, A375 cells, HUVEC cells, UCMSCs cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15675-1-AP targets CD44 in WB, IHC, IF/ICC, IF-P, IP, CoIP, Neutralization, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, bovine, sika deer
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD44 fusion protein Ag8143
Full Name CD44 molecule (Indian blood group)
Calculated Molecular Weight 742 aa, 82 kDa
Observed Molecular Weight 80-95 kDa
GenBank Accession NumberBC004372
Gene Symbol CD44
Gene ID (NCBI) 960
RRIDAB_2076198
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

Protocols

Product Specific Protocols
WB protocol for CD44 antibody 15675-1-APDownload protocol
IHC protocol for CD44 antibody 15675-1-APDownload protocol
IF protocol for CD44 antibody 15675-1-APDownload protocol
IP protocol for CD44 antibody 15675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Nature

ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis.

Authors - Ting Zhang
View Article
IF

Nanomicro Lett

Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling

Authors - Junji Ren
View Article
mouseIF

Nat Commun

Hyperthermia-triggered biomimetic bubble nanomachines

Authors - Junbin Gao
View Article
humanWB

J Clin Invest

LOXL2-induced PEAR1 Ser891 phosphorylation suppresses CD44 degradation and promotes triple-negative breast cancer metastasis

Authors - Yingzhi Shen
View Article
mouseIF

Adv Sci (Weinh)

The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss

Authors - Yuxiang Hu
View Article
humanWB,IHC,IF

Int J Oral Sci

LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma

Authors - Yuantong Liu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Kishor (Verified Customer) (06-23-2019)

This antibody works very good at 1:1000 in WB over night at 4o C and 1:200 for IF in cultured cells.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 and 1:200
  • Cell Tissue Type: Human cell lines