CD38 Polyclonal antibody
CD38 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25284-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, HL-60 cells, Daudi cells, Ramos cells |
| Positive IHC detected in | human tonsillitis tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
The immunogen of 25284-1-AP is CD38 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD38 fusion protein Ag17868 |
| Full Name | CD38 molecule |
| Calculated Molecular Weight | 300 aa, 34 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC007964 |
| Gene Symbol | CD38 |
| Gene ID (NCBI) | 952 |
| RRID | AB_2880007 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD38 antibody 25284-1-AP | Download protocol |
| IHC protocol for CD38 antibody 25284-1-AP | Download protocol |
| FC protocol for CD38 antibody 25284-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol CD38 Multi-Functionality in Oral Squamous Cell Carcinoma: Prognostic Implications, Immune Balance, and Immune Checkpoint. | ||
Cell Commun Signal TRPV2-induced Ca2+-calcineurin-NFAT signaling regulates differentiation of osteoclast in multiple myeloma. | ||
Oncol Rep CD38 is highly expressed and affects the PI3K/Akt signaling pathway in cervical cancer. | ||
PLoS Pathog The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness | ||
Am J Cancer Res Tumor-associated macrophages promoting PD-L1 expression in infiltrating B cells through the CXCL12/CXCR4 axis in human hepatocellular carcinoma | ||
iScience Mechanisms of HIV-mediated blood-brain barrier compromise and leukocyte transmigration under the current antiretroviral era |
