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CD37 Polyclonal antibody

CD37 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21044-1-AP

Synonyms

CD37, CD37 molecule, GP52 40, Leukocyte antigen CD37, Tetraspanin 26, Tspan 26, TSPAN26

Validation Data Gallery

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  • Western Blot (WB) analysis of rat spleen tissue using CD37 Polyclonal antibody (21044-1-AP)

    WB analysis of rat spleen using 21044-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 21044-1-AP (CD37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using CD37 Polyclonal antibody (21044-1-AP)

    WB analysis of human spleen using 21044-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 21044-1-AP (CD37 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CD37 Polyclonal antibody (21044-1-AP)

    WB analysis of HeLa using 21044-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21044-1-AP (CD37 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD37 Polyclonal antibody (21044-1-AP)

    IHC staining of human tonsillitis using 21044-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21044-1-AP (CD37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD37 Polyclonal antibody (21044-1-AP)

    IHC staining of human tonsillitis using 21044-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21044-1-AP (CD37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CD37 Polyclonal antibody (21044-1-AP)

    IHC staining of human spleen using 21044-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen slide using 21044-1-AP (CD37 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inrat spleen tissue, HeLa cells, human spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21044-1-AP is CD37 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD37 fusion protein Ag15384
Full Name CD37 molecule
Calculated Molecular Weight 281 aa, 32 kDa
Observed Molecular Weight 40-52 kDa
GenBank Accession NumberBC110379
Gene Symbol CD37
Gene ID (NCBI) 951
RRIDAB_2878800
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).

Protocols

Product Specific Protocols
WB protocol for CD37 antibody 21044-1-APDownload protocol
IHC protocol for CD37 antibody 21044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

iScience

The clinical features, prognostic significance, and immune heterogeneity of CD37 in diffuse gliomas.

Authors - Xuejun Yan
View Article
mouseIHC

Sci Rep

89Zr-PET imaging to predict tumor uptake of 177Lu-NNV003 anti-CD37 radioimmunotherapy in mouse models of B cell lymphoma.

Authors - Danique Giesen
View Article