Tested Applications
| Positive WB detected in | HEK-293 cells, LNCaP cells, U2OS cells |
| Positive IHC detected in | human prostate cancer tissue, human medulloblastoma tissue, human testis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IHC | See 9 publications below |
| IF | See 3 publications below |
Product Information
14453-1-AP targets B7-H3/CD276 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | B7-H3/CD276 fusion protein Ag6006 Predict reactive species |
| Full Name | CD276 molecule |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC062581 |
| Gene Symbol | CD276 |
| Gene ID (NCBI) | 80381 |
| RRID | AB_2073577 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5ZPR3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for B7-H3/CD276 antibody 14453-1-AP | Download protocol |
| IHC protocol for B7-H3/CD276 antibody 14453-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Nutr Biochem Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression | ||
J Cell Physiol Downregulated miR-187 contributes to the keratinocytes hyperproliferation in psoriasis. | ||
CNS Neurosci Ther PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma. | ||
J Cancer The immunoregulatory protein B7-H3 promotes aerobic glycolysis in oral squamous carcinoma via PI3K/Akt/mTOR pathway. | ||
Med Oncol Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression. | ||
J Oncol Alpha-Fetoprotein Regulates the Expression of Immune-Related Proteins through the NF-κB (P65) Pathway in Hepatocellular Carcinoma Cells. |
