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CD27 Monoclonal antibody

CD27 Monoclonal Antibody for WB, IHC, IF-P, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF-P, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1C1G3

Cat no : 66308-1-Ig

Synonyms

T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD27 Monoclonal antibody (66308-1-Ig)

    WB analysis using 66308-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66308-1-Ig (CD27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig spleen tissue using CD27 Monoclonal antibody (66308-1-Ig)

    WB analysis of pig spleen using 66308-1-Ig

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66308-1-Ig (CD27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IHC staining of human tonsillitis using 66308-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IHC staining of human tonsillitis using 66308-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IF Staining of human tonsillitis using 66308-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD27 Monoclonal antibody (66308-1-Ig)

    IF Staining of human tonsillitis using 66308-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of human PBMCs using CD27 Monoclonal antibody (66308-1-Ig)

    FC experiment of human PBMCs using 66308-1-Ig

    1x10^6 human PBMCs were surface stained with 0.2 μg CD27 Monoclonal antibody (66308-1-Ig, Clone:1C1G3) or 0.2 μg Mouse IgG1 isotype control (66360-1-Ig, Clone: T1F8D3F10), and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO.RGAM005). Cells were not fixed.

Tested Applications

Positive WB detected inRaji cells, pig spleen tissue, Ramos cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

66308-1-Ig targets CD27 in WB, IHC, IF-P, FC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD27 fusion protein Ag24537
Full Name CD27 molecule
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC012160
Gene Symbol CD27
Gene ID (NCBI) 939
RRIDAB_2881690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

Protocols

Product Specific Protocols
WB protocol for CD27 antibody 66308-1-IgDownload protocol
IHC protocol for CD27 antibody 66308-1-IgDownload protocol
IF protocol for CD27 antibody 66308-1-IgDownload protocol
FC protocol for CD27 antibody 66308-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Single-cell and spatial transcriptome analyses reveal tertiary lymphoid structures linked to tumour progression and immunotherapy response in nasopharyngeal carcinoma

Authors - Yang Liu
View Article