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CD226 Polyclonal antibody

CD226 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17842-1-AP

Synonyms

DNAX accessory molecule 1, DNAM-1, DNAM1, DNAM 1, CD226 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD226 Polyclonal antibody (17842-1-AP)

    WB analysis using 17842-1-AP

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 17842-1-AP (CD226 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CD226 Polyclonal antibody (17842-1-AP)

    WB analysis of Jurkat using 17842-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17842-1-AP (CD226 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD226 Polyclonal antibody (17842-1-AP)

    IHC staining of human tonsillitis using 17842-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17842-1-AP (CD226 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHuman peripheral blood platelets, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17842-1-AP targets CD226 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD226 fusion protein Ag12206
Full Name CD226 molecule
Calculated Molecular Weight 336 aa, 39 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC074787
Gene Symbol CD226
Gene ID (NCBI) 10666
RRIDAB_2072623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).

Protocols

Product Specific Protocols
WB protocol for CD226 antibody 17842-1-APDownload protocol
IHC protocol for CD226 antibody 17842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Adv Sci (Weinh)

Deciphering the Molecular Characteristics of Human Idiopathic Nonobstructive Azoospermia from the Perspective of Germ Cells

Authors - Yidong Chen
View Article