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  • Featured Product
  • KD/KO Validated

CD1d Monoclonal antibody

CD1d Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

CloneNo.

1C12F1

Cat no : 66257-1-Ig

Synonyms

CD1A, CD1D, CD1d molecule, R3, R3G1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD1d Monoclonal antibody (66257-1-Ig)

    WB analysis of Jurkat using 66257-1-Ig

    WB result of CD1D antibody (66257-1-Ig, 1:500) with si-Control and si-CD1D transfected Jurkat cells.

  • Western Blot (WB) analysis of human testis tissue using CD1d Monoclonal antibody (66257-1-Ig)

    WB analysis of human testis using 66257-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66257-1-Ig (CD1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using CD1d Monoclonal antibody (66257-1-Ig)

    IHC staining of human skin using 66257-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CD1d Monoclonal antibody (66257-1-Ig)

    IHC staining of human skin using 66257-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CD1d Monoclonal antibody (66257-1-Ig)

    IHC staining of human small intestine using 66257-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CD1d Monoclonal antibody (66257-1-Ig)

    IHC staining of human small intestine using 66257-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inhuman testis tissue, Jurkat
Positive IHC detected inhuman skin tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66257-1-Ig targets CD1d in WB, IHC, ELISA, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD1d fusion protein Ag11108
Full Name CD1d molecule
Calculated Molecular Weight 335 aa, 38 kDa
Observed Molecular Weight 40 kDa, 50-55 kDa
GenBank Accession NumberBC027926
Gene Symbol CD1D
Gene ID (NCBI) 912
RRIDAB_2881645
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-55 kDa.

Protocols

Product Specific Protocols
WB protocol for CD1d antibody 66257-1-IgDownload protocol
IHC protocol for CD1d antibody 66257-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Open Med (Wars)

CD1d affects the proliferation, migration, and apoptosis of human papillary thyroid carcinoma TPC-1 cells via regulating MAPK/NF-κB signaling pathway

Authors - Qingyuan Liu
  • KD Validated
View Article