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CD163 Polyclonal antibody
CD163 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, pig and More (1)
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
175
Cat no : 16646-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig spleen tissue, human placenta tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human tonsillitis tissue, human liver tissue, human placenta tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue, human liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 32 publications below |
| IHC | See 72 publications below |
| IF | See 90 publications below |
| FC | See 5 publications below |
Product Information
16646-1-AP targets CD163 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD163 fusion protein Ag10029 |
| Full Name | CD163 molecule |
| Calculated Molecular Weight | 1156 aa, 125 kDa |
| Observed Molecular Weight | 130-150 kDa |
| GenBank Accession Number | BC051281 |
| Gene Symbol | CD163 |
| Gene ID (NCBI) | 9332 |
| RRID | AB_2756528 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD163 antibody 16646-1-AP | Download protocol |
| IHC protocol for CD163 antibody 16646-1-AP | Download protocol |
| IF protocol for CD163 antibody 16646-1-AP | Download protocol |
| IP protocol for CD163 antibody 16646-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mil Med Res Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease | ||
J Clin Invest CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages | ||
Adv Sci (Weinh) Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis | ||
Cell Rep Med Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response | ||
Nat Commun Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target. | ||
Redox Biol Lactate-induced activation of tumor-associated fibroblasts and IL-8-mediated macrophage recruitment promote lung cancer progression |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Jaouhara (Verified Customer) (12-05-2023) | Works very well in immunohistochemistry (human cartilage)
|
FH Kenzo (Verified Customer) (08-29-2023) | Works well by IF on frozen mouse liver tissue sections.
|
FH Yasuyo (Verified Customer) (05-27-2022) | Works pretty good.
 |
FH Emma (Verified Customer) (11-29-2021) | Works well by IF on FFPE tissue @ 1:100 with a Tris-EDTA antigen retrieval.
 |
