CD16 Polyclonal antibody

CD16 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 16559-1-AP

Synonyms

Fc gamma RIIIa, Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen



Tested Applications

Positive IHC detected inhuman liver tissue, human placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16559-1-AP targets CD16 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD16 fusion protein Ag9787
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated Molecular Weight 254 aa, 29 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC017865
Gene Symbol CD16a
Gene ID (NCBI) 2214
RRIDAB_2878279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.

Protocols

Product Specific Protocols
IHC protocol for CD16 antibody 16559-1-APDownload protocol
IF protocol for CD16 antibody 16559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Immunother Cancer

Discovery of a glucocorticoid receptor (GR) activity signature correlates with immune cell infiltration in adrenocortical carcinoma

Authors - Kan Wu
humanWB

J Allergy Clin Immunol

Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces TH2/TH17 polarization through dectin-2.

Authors - Yunsheng Liang
ratIF

Theranostics

Effects of prenatal photobiomodulation treatment on neonatal hypoxic ischemia in rat offspring.

Authors - Luodan Yang
ratWB,IF

Theranostics

Non-invasive photobiomodulation treatment in an Alzheimer Disease-like transgenic rat model.

Authors - Luodan Yang
humanIF

J Transl Med

Systematic analysis of various RNA transcripts and construction of biological regulatory networks at the post-transcriptional level for chronic obstructive pulmonary disease

Authors - Beibei Li
mouseIHC

Front Immunol

Efficacy of MUC1-targeted CAR-NK cells against human tongue squamous cell carcinoma

Authors - Xiaolan Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH9.0.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Prostate