• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD14 Monoclonal antibody

CD14 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA, Cell treatment

Conjugate

Unconjugated

CloneNo.

2C1D9

Cat no : 60253-1-Ig

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 2C1D9

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CD14 Monoclonal antibody (60253-1-Ig)

    WB analysis using 60253-1-Ig

    Untreated and PNGase F-treated lysates of human milk were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using CD14 Monoclonal antibody (60253-1-Ig)

    WB analysis of THP-1 using 60253-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using CD14 Monoclonal antibody (60253-1-Ig)

    WB analysis of human placenta using 60253-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using CD14 Monoclonal antibody (60253-1-Ig)

    WB analysis of RAW 264.7 using 60253-1-Ig

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD14 Monoclonal antibody (60253-1-Ig)

    IHC staining of human tonsillitis using 60253-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD14 Monoclonal antibody (60253-1-Ig)

    IHC staining of human tonsillitis using 60253-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD14 Monoclonal antibody (60253-1-Ig)

    IF Staining of human tonsillitis using 60253-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD14 antibody (60253-1-Ig, Clone: 2C1D9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD14 Monoclonal antibody (60253-1-Ig)

    IF Staining of RAW 264.7 using 60253-1-Ig

    Immunofluorescent analysis of RAW 264.7 cells using 60253-1-Ig (CD14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Tested Applications

Positive WB detected inTHP-1 cells, human milk, human placenta tissue, RAW 264.7 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inRAW 264.7 cells, human tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60253-1-Ig targets CD14 in WB, IHC, IF/ICC, IF-P, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD14 fusion protein Ag10693
Full Name CD14 molecule
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 45-55 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
RRIDAB_2881374
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
WB protocol for CD14 antibody 60253-1-IgDownload protocol
IHC protocol for CD14 antibody 60253-1-IgDownload protocol
IF protocol for CD14 antibody 60253-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Mol Cell

Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells.

Authors - Jia Xiong
View Article
humanIF

Arthritis Rheumatol

Attenuation of rheumatoid arthritis through the inhibition of caspase3/GSDME-mediated pyroptosis induced by TNF-α.

Authors - Zeqing Zhai
View Article
rabbitIF

Bioact Mater

Novel magnetic silk fibroin scaffolds with delayed degradation for potential long-distance vascular repair.

Authors - Xin Liu
View Article
mouseWB,IF

Sci Rep

High-Throughput Sequencing and Co-Expression Network Analysis of lncRNAs and mRNAs in Early Brain Injury Following Experimental Subarachnoid Haemorrhage.

Authors - Jianhua Peng
View Article
mouseCell treatment

Front Pharmacol

Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway

Authors - Sairong Fan
View Article
humanIHC

J Cancer

MMP9 and IGFBP1 Regulate Tumor Immune and Drive Tumor Progression in Clear Cell Renal Cell Carcinoma.

Authors - Tianbo Xu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Sareena (Verified Customer) (06-06-2023)

Works well when conjugated to fluorophores and tested with compensation beads

  • Applications: Immunofluorescence, Flow Cytometry
  • Primary Antibody Dilution: CD14 Monoclonal antibody
FH

Sunil (Verified Customer) (05-15-2023)

Great and fast delivery which is always useful!

  • Applications: Flow Cytometry
FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Tris-EDTA antigen retrieval pH 9.0.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
CD14 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:60253-1-Ig)