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CD109 Monoclonal antibody

CD109 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H8G7

Cat no : 60179-1-Ig

Synonyms

CD109, CD109 antigen, CD109 molecule, CPAMD7, p180, Platelet specific Gov antigen, r150, RP11 525G3.1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD109 Monoclonal antibody (60179-1-Ig)

    WB analysis of Jurkat using 60179-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60179-1-Ig (CD109 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60179-1-Ig targets CD109 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name CD109 molecule
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberBC152996
Gene Symbol CD109
Gene ID (NCBI) 135228
RRIDAB_10892668
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD109, also named as CPAMD7, is a member of the alpha2-macroglobulin/complement superfamily. It is a glycosylphosphatidylinositol (GPI)-linked cell surface glycoprotein of approximately 170kd which is found on the cell surface of platelets, activated T-cells, and endothelial cells. CD109 binds to and negatively regulates signaling of transforming growth factor beta (TGF-beta). CD109 has been identified as part of the TGF-beta receptor system in human keratinocytes. Up regulation of CD109 expression has been observed in several different types of tumor.CD109 is a glycosyl-phosphatidylinositol-anchored glycoprotein that is a member of the a2-macroglobulin/C3, C4, C5 family of thioester-containing proteins. There're 2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form. The antibody can recognize all these 4 isoforms.

Protocols

Product Specific Protocols
WB protocol for CD109 antibody 60179-1-IgDownload protocol
FC protocol for CD109 antibody 60179-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Oral Implants Res

Characterization of macrophages infiltrating peri-implantitis lesions.

Authors - Tobias Fretwurst
View Article

Drug Dev Res

Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression.

Authors - Junyu Fan
View Article
humanIHC

Mol Carcinog

Tandem mass tag (TMT) quantitative protein analysis-based proteomics and parallel reaction monitoring (PRM) validation revealed that MST4 accelerates osteosarcoma proliferation by increasing MRC2 activity

Authors - Yunyan Liu
View Article