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CCR10 Polyclonal antibody

CCR10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22071-1-AP

Synonyms

CC-CKR-10, C-C CKR-10, CC CKR 10, C-C chemokine receptor type 10, C C CKR 10

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CCR10 Polyclonal antibody (22071-1-AP)

    WB analysis of mouse brain using 22071-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22071-1-AP (CCR10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CCR10 Polyclonal antibody (22071-1-AP)

    WB analysis of rat brain using 22071-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 22071-1-AP (CCR10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CCR10 Polyclonal antibody (22071-1-AP)

    IHC staining of human spleen using 22071-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 22071-1-AP (CCR10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CCR10 Polyclonal antibody (22071-1-AP)

    IHC staining of human spleen using 22071-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 22071-1-AP (CCR10 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22071-1-AP targets CCR10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCR10 fusion protein Ag17212
Full Name chemokine (C-C motif) receptor 10
Calculated Molecular Weight 362 aa, 38 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC099840
Gene Symbol CCR10
Gene ID (NCBI) 2826
RRIDAB_11182921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR10 (also known as GPR2) is a G-protein coupled receptor for chemokines CCL27 (SCYA27) and CCL28 (SCYA28). CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation (PMID: 11821900). CCR10-CCL28 interactions are thought to contribute to the accumulation of IgA Ab-secreting cells (ASC) on mucosal surfaces.

Protocols

Product Specific Protocols
WB protocol for CCR10 antibody 22071-1-APDownload protocol
IHC protocol for CCR10 antibody 22071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,FC

Adv Sci (Weinh)

Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression

Authors - Rui Zhou
View Article
humanWB

Front Immunol

Single-Cell Sequencing Reveals the Transcriptome and TCR Characteristics of pTregs and in vitro Expanded iTregs.

Authors - Zhenzhen Hui
View Article
humanIHC

Mol Med Rep

Knockdown of CCL28 inhibits endometriosis stromal cell proliferation and invasion via ERK signaling pathway inactivation

Authors - YINGTING WU
View Article
mouseIHC

Inflamm Regen

Th22 is the effector cell of thymosin β15-induced hair regeneration in mice

Authors - Nana Tao
View Article