Cyclin T1 Polyclonal antibody

Cyclin T1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20992-1-AP

Synonyms

CCNT, CCNT1, Cyclin T, cyclin T1, CYCT1, HIVE1



Tested Applications

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells, Y79 cells, A431 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20992-1-AP targets Cyclin T1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cyclin T1
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberNM_001240
Gene Symbol CCNT1
Gene ID (NCBI) 904
RRIDAB_10950894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNT1, also named as CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.

Protocols

Product Specific Protocols
WB protocol for Cyclin T1 antibody 20992-1-APDownload protocol
IF protocol for Cyclin T1 antibody 20992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Authors - Gongwei Wu
humanWB

Nucleic Acids Res

HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.

Authors - Sebastian Eilebrecht
humanWB,IF

BMC Cancer

The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study

Authors - Ying E