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CCNI2 Polyclonal antibody

CCNI2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18822-1-AP

Synonyms

CCNI2, cyclin I family, member 2, Cyclin I2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCNI2 Polyclonal antibody (18822-1-AP)

    WB analysis using 18822-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18822-1-AP (CCNI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CCNI2 Polyclonal antibody (18822-1-AP)

    WB analysis of human brain using 18822-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18822-1-AP (CCNI2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CCNI2 Polyclonal antibody (18822-1-AP)

    WB analysis using 18822-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 18822-1-AP (CCNI2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CCNI2 Polyclonal antibody (18822-1-AP)

    IHC staining of human lung cancer using 18822-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 18822-1-AP (CCNI2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CCNI2 Polyclonal antibody (18822-1-AP)

    IHC staining of human lung cancer using 18822-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 18822-1-AP (CCNI2 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, human brain tissue, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18822-1-AP targets CCNI2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cyclin I family, member 2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC144417
Gene Symbol CCNI2
Gene ID (NCBI) 645121
RRIDAB_10596930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNI2 belongs to the cyclin family.

Protocols

Product Specific Protocols
WB protocol for CCNI2 antibody 18822-1-APDownload protocol
IHC protocol for CCNI2 antibody 18822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Sci Rep

Cyclin I-like (CCNI2) is a cyclin-dependent kinase 5 (CDK5) activator and is involved in cell cycle regulation.

Authors - Chengcheng Liu
View Article