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CBS Polyclonal antibody

Name: CBS Polyclonal antibody
Brand: Proteintech
SKU: 14787-1-AP

CBS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

110

Review Product

Cat no : 14787-1-AP

Synonyms

HIP4, EC:4.2.1.22, Cystathionine beta-synthase, cystathionine beta synthase, Beta-thionase

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 14787-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of NCI-H1299 using 14787-1-AP
WB result of CBS antibody (14787-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBS transfected NCI-H1299 cells.
WB analysis using 14787-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 14787-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14787-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 14787-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 14787-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 14787-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14787-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 14787-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 14787-1-AP
IP result of anti-CBS (IP:14787-1-AP, 3ug; Detection:14787-1-AP 1:500) with mouse kidney tissue lysate 4000ug.
IHC staining of human pancreas cancer using 14787-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 14787-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 14787-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14787-1-AP (CBS antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human pancreas cancer using 14787-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14787-1-AP (CBS antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human colon using 14787-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 10x lens).
IF Staining of HeLa using 14787-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CBS antibody (14787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 14787-1-AP

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Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, mouse colon tissue, NCI-H1299 cells, rat brain tissue, rat kidney tissue, rat liver tissue, HepG2 cells, LNCaP cells, MCF-7 cells, SKOV-3 cells, THP-1 cells, mouse kidney tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human pancreas cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 98 publications below
IHC	See 18 publications below
IF	See 16 publications below

Product Information

14787-1-AP targets CBS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CBS fusion protein Ag6437
Full Name	cystathionine-beta-synthase
Calculated Molecular Weight	61 kDa
Observed Molecular Weight	61-63 kDa
GenBank Accession Number	BC000440
Gene Symbol	CBS
Gene ID (NCBI)	875
RRID	AB_2070970
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).

Protocols

Product Specific Protocols
WB protocol for CBS antibody 14787-1-AP	Download protocol
IHC protocol for CBS antibody 14787-1-AP	Download protocol
IF protocol for CBS antibody 14787-1-AP	Download protocol
IP protocol for CBS antibody 14787-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. Authors - Hagai Marmor-Kollet View Article
human	WB	Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis. Authors - Elodie Villa View Article
canine	IF	Nat Commun Remote ischemic conditioning counteracts the intestinal damage of necrotizing enterocolitis by improving intestinal microcirculation. Authors - Yuhki Koike View Article
mouse	WB,IHC,IF	Redox Biol Endogenous hydrogen sulfide accelerated trauma-induced heterotopic ossification through the Ca2+/ERK pathway-enhanced aberrant osteogenic activity Authors - Zhengqiang Yuan View Article
human	WB	Cancer Res PRMT1 sustains de novo fatty acid synthesis by methylating PHGDH to drive chemoresistance in triple-negative breast cancer Authors - Takehiro Yamamoto View Article
mouse	WB	Redox Biol Cystathionine γ-lyase promotes estrogen-stimulated uterine artery blood flow via glutathione homeostasis. Authors - Rachael Bok View Article

CBS Polyclonal antibody

CBS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 14787-1-AP

WB analysis of NCI-H1299 using 14787-1-AP

WB analysis using 14787-1-AP

WB analysis of rat liver using 14787-1-AP

WB analysis of HeLa using 14787-1-AP

WB analysis using 14787-1-AP

WB analysis of rat brain using 14787-1-AP

WB analysis of mouse colon using 14787-1-AP

WB analysis of HEK-293 using 14787-1-AP

WB analysis of rat kidney using 14787-1-AP

IP experiment of mouse kidney using 14787-1-AP

IHC staining of human pancreas cancer using 14787-1-AP

IHC staining of human pancreas cancer using 14787-1-AP

IHC staining of human pancreas cancer using 14787-1-AP

IHC staining of human pancreas cancer using 14787-1-AP

IHC staining of human colon using 14787-1-AP

IF Staining of HeLa using 14787-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Remote ischemic conditioning counteracts the intestinal damage of necrotizing enterocolitis by improving intestinal microcirculation.

Endogenous hydrogen sulfide accelerated trauma-induced heterotopic ossification through the Ca2+/ERK pathway-enhanced aberrant osteogenic activity

PRMT1 sustains de novo fatty acid synthesis by methylating PHGDH to drive chemoresistance in triple-negative breast cancer

Cystathionine γ-lyase promotes estrogen-stimulated uterine artery blood flow via glutathione homeostasis.

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