• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CBFA2T2 Polyclonal antibody

CBFA2T2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 11336-1-AP

Synonyms

CBFA2T2, EHT, MTG8 like protein, MTG8 related protein 1, MTGR1, p85, Protein CBFA2T2, ZMYND3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using CBFA2T2 Polyclonal antibody (11336-1-AP)

    WB analysis of HEK-293 using 11336-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11336-1-AP (CBFA2T2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CBFA2T2 Polyclonal antibody (11336-1-AP)

    WB analysis of mouse testis using 11336-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11336-1-AP (CBFA2T2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CBFA2T2 Polyclonal antibody (11336-1-AP)

    WB analysis of HeLa using 11336-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11336-1-AP (CBFA2T2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CBFA2T2 Polyclonal antibody (11336-1-AP)

    IHC staining of human gliomas using 11336-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11336-1-AP (CBFA2T2 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse testis tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

ChIPSee 1 publications below

Product Information

11336-1-AP targets CBFA2T2 in WB, IHC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBFA2T2 fusion protein Ag1880
Full Name core-binding factor, runt domain, alpha subunit 2; translocated to, 2
Calculated Molecular Weight 604 aa, 67 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC015066
Gene Symbol CBFA2T2
Gene ID (NCBI) 9139
RRIDAB_2070571
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Core-binding factor, runt domain, alpha subunit 2, translocated to 2(CBFA2T2) belongs to a family of ubiquitously expressed transcriptional repressors that interact with transcription factors bound to promoters of target genes and with histone deacetylases (HDACs) critical for enforcement of repressor function.CBFA2T2, also named as MTGR1 or EHT, is an 85-kDa phosphoprotein. It can bind AML1-MTG8 fusion protein in acute myeloid leukemia. The binding leads to an enhancement of cell proliferation mediated in a murine myeloid model. The calculated molecular weight of CBFA2T2 is 67 kDa, but modified SMARCE1 is about 80 kDa.

Protocols

Product Specific Protocols
WB protocol for CBFA2T2 antibody 11336-1-APDownload protocol
IHC protocol for CBFA2T2 antibody 11336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chIP

Oncogene

Myeloid translocation genes differentially regulate colorectal cancer programs.

Authors - B Parang
View Article