Caspase 9 AntibodyRabbit Polyclonal
|Tested applications||ELISA, IF, IHC, IP, WB|
|Species specificity||Human,Mouse,Rat; other species not tested.|
|Cited species||human, mouse, rat|
|Positive WB detected in||HeLa cells, HL-60 cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse lung tissue, human heart tissue|
|Positive IF detected in||HepG2 cells|
|Recommended dilution||WB: 1:500-1:5000|
IP = Immunoprecipitation
- HeLa cells were subjected to SDS PAGE followed by western blot with 10380-1-AP(CASP9 antibody) at dilution of 1:1000
- IP Result of anti-CASP9 (IP:10380-1-AP, 3ug; Detection:10380-1-AP 1:200) with HeLa cells lysate 2500ug.
- Immunohistochemical of paraffin-embedded mouse lung using 10380-1-AP(CASP9 antibody) at dilution of 1:25 (under 10x lens)
- Immunohistochemical of paraffin-embedded mouse lung using 10380-1-AP(CASP9 antibody) at dilution of 1:25 (under 40x lens)
- Immunofluorescent analysis of HepG2 cells, using CASP9 antibody 10380-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||Caspase 9 fusion protein ag0404||Full name||caspase 9, apoptosis-related cysteine peptidase|
|BC002452||Gene ID (NCBI)||842|
|Gene symbol||CASP9||Synonyms||APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, CASPASE 9c, Caspase9, Caspase-9, ICE LAP6, ICE like apoptotic protease 6, MCH6|
Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. CASP9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.