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  • Featured Product
  • KD/KO Validated

CAPRIN1 Polyclonal antibody

CAPRIN1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 15112-1-AP

Synonyms

GPI-anchored protein p137, GPI-anchored membrane protein 1, Cytoplasmic activation- and proliferation-associated protein 1, Cell cycle-associated protein 1, Caprin-1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CAPRIN1 Polyclonal antibody (15112-1-AP)

    WB analysis using 15112-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CAPRIN1 Polyclonal antibody (15112-1-AP)

    WB analysis using 15112-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CAPRIN1 Polyclonal antibody (15112-1-AP)

    WB analysis using 15112-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IP experiment of HeLa using 15112-1-AP

    IP result of anti-CAPRIN1 (IP:15112-1-AP, 4ug; Detection:15112-1-AP 1:5000) with HeLa cells lysate 1600 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IHC staining of human breast cancer using 15112-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IHC staining of human breast cancer using 15112-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IHC staining of mouse testis using 15112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IHC staining of mouse testis using 15112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IHC staining of human prostate cancer using 15112-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IHC staining of human testis using 15112-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 15112-1-AP (CAPRIN1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IHC staining of human testis using 15112-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 15112-1-AP (CAPRIN1 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IF Staining of HepG2 using 15112-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IF Staining of HeLa using 15112-1-AP

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CAPRIN1 antibody (15112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of OC2 cells using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IF Staining of OC2 cells using 15112-1-AP

    IF result of anti-CAPRIN1 (15112-1-AP, 1:200) with OC2 cells by Dr. Emily Towers.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IF Staining of HeLa using 15112-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAPRIN1 antibody (15112-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of cochlear cryosections using CAPRIN1 Polyclonal antibody (15112-1-AP)

    IF Staining of cochlear cryosections using 15112-1-AP

    IF result of anti-CAPRIN1 (15112-1-AP, 1:200) with Cochlear cryosections by Dr. Emily Towers. CAPRIN1, purple; DAPI, blue.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CAPRIN1 Polyclonal antibody (15112-1-AP)

    FC experiment of NIH/3T3 using 15112-1-AP

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CAPRIN1 (15112-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, HEK-293T cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, sodium arsenite treated HeLa cells, HepG2 cells, OC2 cells, cochlear cryosections
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15112-1-AP targets CAPRIN1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAPRIN1 fusion protein Ag7099
Full Name cell cycle associated protein 1
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC001731
Gene Symbol CAPRIN1
Gene ID (NCBI) 4076
RRIDAB_2070016
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

Protocols

Product Specific Protocols
WB protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IHC protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IF protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IP protocol for CAPRIN1 antibody 15112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
View Article
humanWB

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang
  • KD Validated
View Article
humanWB,IF

Cell

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Authors - David W Sanders
View Article
humanWB,IF

Nat Immunol

Inducible degradation of lncRNA Sros1 promotes IFN-γ-mediated activation of innate immune responses by stabilizing Stat1 mRNA.

Authors - Henan Xu
View Article
mouseWB,RIP

Nat Commun

Fear extinction is regulated by the activity of long noncoding RNAs at the synapse

Authors - Wei-Siang Liau
View Article
WB, IP

Neuron

CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to ensure proper dendritic spine formation.

Authors - Silvia De Rubeis
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Katherine (Verified Customer) (10-31-2023)

The antibody worked well with 1:5000 dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: 293T
FH

Lisa (Verified Customer) (02-20-2020)

The antibody works very well, giving specific bands in WB and a very good staining in IF.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: WB:1:2500 - IF:1:100
  • Cell Tissue Type: Fibroblasts, lymphoblastoid cell line
CAPRIN1 Antibody Western Blot,Immunofluorescence, validation (WB:1:2500 - IF:1:100 dilution) in Fibroblasts, lymphoblastoid cell line (Cat no:15112-1-AP)
FH

Paul (Verified Customer) (04-30-2019)

Product works very well for WB, IP and IF using rat brain tissue samples.

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Rat brain
CAPRIN1 Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:2000 dilution) in Rat brain (Cat no:15112-1-AP)