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Calpain S2 Polyclonal antibody

Calpain S2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15803-1-AP

Synonyms

CAPNS2, CSS2, calpain, small subunit 2, Calpain small subunit 2, Calcium-dependent protease small subunit 2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using Calpain S2 Polyclonal antibody (15803-1-AP)

    WB analysis of mouse skin using 15803-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 15803-1-AP (Calpain S2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skin tissue using Calpain S2 Polyclonal antibody (15803-1-AP)

    WB analysis of rat skin using 15803-1-AP

    rat skin tissue were subjected to SDS PAGE followed by western blot with 15803-1-AP (Calpain S2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skin tissue using Calpain S2 Polyclonal antibody (15803-1-AP)

    IP experiment of mouse skin using 15803-1-AP

    IP result of anti-Calpain S2 (IP:15803-1-AP, 4ug; Detection:15803-1-AP 1:300) with mouse skin tissue lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Calpain S2 Polyclonal antibody (15803-1-AP)

    IHC staining of human skin cancer using 15803-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15803-1-AP (Calpain S2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Calpain S2 Polyclonal antibody (15803-1-AP)

    IHC staining of human skin cancer using 15803-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15803-1-AP (Calpain S2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse skin tissue, rat skin tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15803-1-AP targets Calpain S2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calpain S2 fusion protein Ag8534
Full Name calpain, small subunit 2
Calculated Molecular Weight 248 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC005397
Gene Symbol Calpain S2
Gene ID (NCBI) 84290
RRIDAB_2259580
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calpain S2, also known as calpain-2, is a member of the calpain family of calcium-dependent proteases that play crucial roles in various cellular processes.

Protocols

Product Specific Protocols
WB protocol for Calpain S2 antibody 15803-1-APDownload protocol
IHC protocol for Calpain S2 antibody 15803-1-APDownload protocol
IP protocol for Calpain S2 antibody 15803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols