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Calpain 11 Polyclonal antibody

Calpain 11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13675-1-AP

Synonyms

EC:3.4.22.-, CAPN11, CANP 11, Calpain-11, calpain11

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using Calpain 11 Polyclonal antibody (13675-1-AP)

    WB analysis of PC-3 using 13675-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13675-1-AP (Calpain 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Calpain 11 Polyclonal antibody (13675-1-AP)

    IHC staining of mouse testis using 13675-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13675-1-AP (Calpain 11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using Calpain 11 Polyclonal antibody (13675-1-AP)

    IF Staining of PC-3 using 13675-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13675-1-AP (Calpain 11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13675-1-AP targets Calpain 11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calpain 11 fusion protein Ag4668
Full Name calpain 11
Calculated Molecular Weight 702 aa, 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC033733
Gene Symbol Calpain 11
Gene ID (NCBI) 11131
RRIDAB_2069480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calpains are a superfamily of related proteins, some of which have been shown to function as intracellular calcium-dependent cysteine proteases. Calpains consist of a variable large (80 kDa) subunit and an invariant small (30 kDa) subunit. The expression of calpain11 during spermatogenesis and its localization is spermatozoa suggest that it is involved in regulating calcium-dependent signal transduction events during meiosis and sperm functional processes(PMID:16541461). And the 110 kDa band protein may be regarded as a complex.

Protocols

Product Specific Protocols
WB protocol for Calpain 11 antibody 13675-1-APDownload protocol
IHC protocol for Calpain 11 antibody 13675-1-APDownload protocol
IF protocol for Calpain 11 antibody 13675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols