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  • Featured Product
  • KD/KO Validated

Calumenin Polyclonal antibody

Calumenin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17804-1-AP

Synonyms

CALU, Calumenin, Crocalbin, IEF SSP 9302

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Calumenin Polyclonal antibody (17804-1-AP)

    WB analysis of HEK-293 using 17804-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using Calumenin Polyclonal antibody (17804-1-AP)

    WB analysis of mouse kidney using 17804-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using Calumenin Polyclonal antibody (17804-1-AP)

    WB analysis of K-562 using 17804-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Calumenin Polyclonal antibody (17804-1-AP)

    WB analysis of HepG2 using 17804-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Calumenin Polyclonal antibody (17804-1-AP)

    WB analysis of human placenta using 17804-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Calumenin Polyclonal antibody (17804-1-AP)

    IHC staining of human placenta using 17804-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Calumenin Polyclonal antibody (17804-1-AP)

    IHC staining of human placenta using 17804-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Calumenin Polyclonal antibody (17804-1-AP)

    IHC staining of mouse brain using 17804-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Calumenin Polyclonal antibody (17804-1-AP)

    IHC staining of mouse brain using 17804-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Calumenin Polyclonal antibody (17804-1-AP)

    IF Staining of HeLa using 17804-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calumenin antibody (17804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, human placenta tissue, K-562 cells, mouse kidney tissue
Positive IHC detected inhuman placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17804-1-AP targets Calumenin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calumenin fusion protein Ag12073
Full Name calumenin
Calculated Molecular Weight 315 aa, 37 kDa
Observed Molecular Weight 47 kDa, 57 kDa
GenBank Accession NumberBC013383
Gene Symbol Calumenin
Gene ID (NCBI) 813
RRIDAB_2069964
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Calumenin antibody 17804-1-APDownload protocol
IHC protocol for Calumenin antibody 17804-1-APDownload protocol
IF protocol for Calumenin antibody 17804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
View Article
humanWB

Oncogene

Extracellular calumenin suppresses ERK1/2 signaling and cell migration by protecting fibulin-1 from MMP-13-mediated proteolysis.

Authors - Q Wang
  • KD Validated
View Article
humanIF

Cancer Med

Five EMT-related genes signature predicts overall survival and immune environment in microsatellite instability-high gastric cancer.

Authors - Mili Zhang
View Article
pigWB

Cell Tissue Res

Hormone-regulated PKA activity in porcine oviductal epithelial cells.

Authors - Juan Manuel Teijeiro
View Article
humanWB,IHC,IF

J Inflamm Res

Pan-Cancer Screening and Validation of CALU's Role in EMT Regulation and Tumor Microenvironment in Triple-Negative Breast Cancer

Authors - Shi-Liang Chen
View Article