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CALML5 Polyclonal antibody

CALML5 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13059-1-AP

Synonyms

CALML5, calmodulin like 5, Calmodulin like protein 5, Calmodulin like skin protein, CLSP

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CALML5 Polyclonal antibody (13059-1-AP)

    IHC staining of human skin cancer using 13059-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13059-1-AP (CALML5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CALML5 Polyclonal antibody (13059-1-AP)

    IHC staining of human skin cancer using 13059-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13059-1-AP (CALML5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CALML5 Polyclonal antibody (13059-1-AP)

    IHC staining of human breast cancer using 13059-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13059-1-AP (CALML5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CALML5 Polyclonal antibody (13059-1-AP)

    IHC staining of human breast cancer using 13059-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13059-1-AP (CALML5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman skin cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13059-1-AP targets CALML5 in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALML5 fusion protein Ag3734
Full Name calmodulin-like 5
Calculated Molecular Weight 146 aa, 16 kDa
GenBank Accession NumberBC039172
Gene Symbol CALML5
Gene ID (NCBI) 51806
RRIDAB_2069463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CALML5, also known as calmodulin-like skin protein (CLSP), a recently discovered skin-specific calcium-binding protein, is closely related to keratinocyte differentiation. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes.

Protocols

Product Specific Protocols
IHC protocol for CALML5 antibody 13059-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Ann Diagn Pathol

Loss of ELF3 immunoexpression is useful for detecting oral squamous cell carcinoma but not for distinguishing between grades of epithelial dysplasia.

Authors - Abdulmajeed Ahmad A AA
View Article
humanIHC

Elife

Dalpiciclib partially abrogates ER signaling activation induced by pyrotinib in HER2+HR+ breast cancer

Authors - Jiawen Bu
View Article