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  • Featured Product
  • KD/KO Validated

CALML4 Polyclonal antibody

CALML4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 15894-1-AP

Synonyms

CALML4, calmodulin like 4, Calmodulin like protein 4, NY BR 20

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CALML4 Polyclonal antibody (15894-1-AP)

    WB analysis using 15894-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15894-1-AP (CALML4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CALML4 Polyclonal antibody (15894-1-AP)

    WB analysis of mouse thymus using 15894-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15894-1-AP (CALML4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using CALML4 Polyclonal antibody (15894-1-AP)

    WB analysis of human colon using 15894-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 15894-1-AP (CALML4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse thymus tissue using CALML4 Polyclonal antibody (15894-1-AP)

    IP experiment of mouse thymus using 15894-1-AP

    IP result of anti-CALML4 (IP:15894-1-AP, 4ug; Detection:15894-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

Tested Applications

Positive WB detected inmouse kidney tissue, mouse thymus tissue, human colon tissue, rat kidney tissue
Positive IP detected inmouse thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15894-1-AP targets CALML4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALML4 fusion protein Ag8705
Full Name calmodulin-like 4
Calculated Molecular Weight 196 aa, 22 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC009516
Gene Symbol CALML4
Gene ID (NCBI) 91860
RRIDAB_2069462
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CALML4 antibody 15894-1-APDownload protocol
IP protocol for CALML4 antibody 15894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

ACS Chem Neurosci

Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine.

Authors - Lian Liu
View Article
humanWB,IF

J Biol Chem

The small EF-hand protein CALML4 functions as a critical myosin light chain within the intermicrovillar adhesion complex.

Authors - Myoung Soo Choi
  • KD Validated
View Article
humanWB

J Biol Chem

Molecular Regulatory Mechanism of Human Myosin-7a

Authors - Alexandra Holló
View Article