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KD/KO Validated

NDP52 Polyclonal antibody

NDP52 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12229-1-AP

Synonyms

CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 12229-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 12229-1-AP
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12229-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 12229-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12229-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12229-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 12229-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 12229-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 12229-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 12229-1-AP (NDP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12229-1-AP
IP result of anti-NDP52 (IP:12229-1-AP, 3ug; Detection:12229-1-AP 1:1000) with HeLa cells lysate 2150ug.
IHC staining of human ovary cancer using 12229-1-AP
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12229-1-AP (NDP52 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 12229-1-AP
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12229-1-AP (NDP52 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 12229-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12229-1-AP (NDP52 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 12229-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12229-1-AP (NDP52 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary tumor using 12229-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12229-1-AP (NDP52 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 12229-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12229-1-AP (NDP52 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary tumor using 12229-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12229-1-AP (NDP52 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 12229-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12229-1-AP (NDP52 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12229-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NDP52 antibody (12229-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 12229-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, rat skeletal muscle tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary cancer tissue, human ovary tumor tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 44 publications below
IF	See 3 publications below
IP	See 4 publications below

Product Information

12229-1-AP targets NDP52 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NDP52 fusion protein Ag2866
Full Name	calcium binding and coiled-coil domain 2
Calculated Molecular Weight	446 aa, 52 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	BC015893
Gene Symbol	NDP52
Gene ID (NCBI)	10241
RRID	AB_11182600
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

Protocols

Product Specific Protocols
WB protocol for NDP52 antibody 12229-1-AP	Download protocol
IHC protocol for NDP52 antibody 12229-1-AP	Download protocol
IF protocol for NDP52 antibody 12229-1-AP	Download protocol
IP protocol for NDP52 antibody 12229-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria. Authors - Zhenyu Zhong View Article
human	WB	Mol Cell PMID: 28965816 Authors - Shouheng Jin KO Validated View Article
human	WB	Autophagy TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression Authors - Tai-Mei Zhang View Article
human	WB	Nat Commun TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro. Authors - Minwoo Baek View Article
mouse	WB	Nat Commun Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration. Authors - Insup Choi View Article
mouse	WB	Redox Biol A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease Authors - Yi Sun View Article

NDP52 Polyclonal antibody

NDP52 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 12229-1-AP

WB analysis of rat skeletal muscle using 12229-1-AP

WB analysis of Jurkat using 12229-1-AP

WB analysis of mouse skeletal muscle using 12229-1-AP

WB analysis of HeLa using 12229-1-AP

WB analysis of Jurkat using 12229-1-AP

WB analysis of human placenta using 12229-1-AP

WB analysis of human placenta using 12229-1-AP

WB analysis of human placenta using 12229-1-AP

IP experiment of HeLa using 12229-1-AP

IHC staining of human ovary cancer using 12229-1-AP

IHC staining of human ovary cancer using 12229-1-AP

IHC staining of human skeletal muscle using 12229-1-AP

IHC staining of human skeletal muscle using 12229-1-AP

IHC staining of human ovary tumor using 12229-1-AP

IHC staining of human ovary tumor using 12229-1-AP

IHC staining of human ovary tumor using 12229-1-AP

IHC staining of human ovary tumor using 12229-1-AP

IF Staining of HeLa using 12229-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.

PMID: 28965816

TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression

TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro.

Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.

A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease

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