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C7orf10 Polyclonal antibody

C7orf10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21589-1-AP

Synonyms

C7orf10, DERP13, ORF19

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using C7orf10 Polyclonal antibody (21589-1-AP)

    WB analysis using 21589-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C7orf10 Polyclonal antibody (21589-1-AP)

    WB analysis of HepG2 using 21589-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using C7orf10 Polyclonal antibody (21589-1-AP)

    WB analysis of mouse skeletal muscle using 21589-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using C7orf10 Polyclonal antibody (21589-1-AP)

    WB analysis of HEK-293 using 21589-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using C7orf10 Polyclonal antibody (21589-1-AP)

    WB analysis of mouse pancreas using 21589-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21589-1-AP (C7orf10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using C7orf10 Polyclonal antibody (21589-1-AP)

    IHC staining of human kidney using 21589-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using C7orf10 Polyclonal antibody (21589-1-AP)

    IHC staining of human kidney using 21589-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using C7orf10 Polyclonal antibody (21589-1-AP)

    IHC staining of human pancreas using 21589-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using C7orf10 Polyclonal antibody (21589-1-AP)

    IHC staining of human pancreas using 21589-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 21589-1-AP (C7orf10 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse kidney tissue, mouse pancreas tissue, mouse skeletal muscle tissue, HepG2 cells, HEK-293 cells, rat kidney tissue
Positive IHC detected inhuman kidney tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

21589-1-AP targets C7orf10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C7orf10 fusion protein Ag16159
Full Name chromosome 7 open reading frame 10
Calculated Molecular Weight 471 aa, 51 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC098318
Gene Symbol C7orf10
Gene ID (NCBI) 79783
RRIDAB_10734435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C7orf10 is also named as SUGCT, DERP13 and belongs to the CaiB/BaiF CoA-transferase family. It may function as part of a multiunit enzyme complex (A), similar to the alpha-ketoglutarate dehydrogenase system, or as the second of two independent enzymes, the first of which decarboxylates alpha-ketoadipate to glutarate (B). This protein has 4 isoforms produced by alternative splicing with the molecular weight from 43 kDa to 51 kDa.

Protocols

Product Specific Protocols
WB protocol for C7orf10 antibody 21589-1-APDownload protocol
IHC protocol for C7orf10 antibody 21589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Mol Life Sci

Knockout of the non-essential gene SUGCT creates diet-linked, age-related microbiome disbalance with a diabetes-like metabolic syndrome phenotype.

Authors - Joanna Niska-Blakie
View Article
humanWB

Front Aging Neurosci

A Y374X TDP43 truncation leads to an altered metabolic profile in amyotrophic lateral sclerosis fibroblasts driven by pyruvate and TCA cycle intermediate alterations

Authors - Scott P Allen
View Article