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C5orf44 Polyclonal antibody

C5orf44 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20444-1-AP

Synonyms

UPF0533 protein C5orf44, TRAPPC13, Trafficking protein particle complex subunit 13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C5orf44 Polyclonal antibody (20444-1-AP)

    WB analysis using 20444-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20444-1-AP (C5orf44 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using C5orf44 Polyclonal antibody (20444-1-AP)

    WB analysis of HeLa using 20444-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20444-1-AP (C5orf44 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using C5orf44 Polyclonal antibody (20444-1-AP)

    IHC staining of human kidney using 20444-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using C5orf44 Polyclonal antibody (20444-1-AP)

    IHC staining of human kidney using 20444-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C5orf44 Polyclonal antibody (20444-1-AP)

    IHC staining of human testis using 20444-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C5orf44 Polyclonal antibody (20444-1-AP)

    IHC staining of human testis using 20444-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C5orf44 Polyclonal antibody (20444-1-AP)

    IF Staining of HEK-293 using 20444-1-AP

    Immunofluorescent analysis of HEK-293 cells using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20444-1-AP targets C5orf44 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C5orf44 fusion protein Ag14267
Full Name chromosome 5 open reading frame 44
Calculated Molecular Weight 417 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC012006
Gene Symbol C5orf44
Gene ID (NCBI) 80006
RRIDAB_10697663
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of C5orf44 has not been widely studied, and is yet to be fully elucidated. The MW of this protein is 47 kDa, and this antibody specially recognises the 47 kDa protein.

Protocols

Product Specific Protocols
WB protocol for C5orf44 antibody 20444-1-APDownload protocol
IHC protocol for C5orf44 antibody 20444-1-APDownload protocol
IF protocol for C5orf44 antibody 20444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols