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C5orf4 Polyclonal antibody

C5orf4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22046-1-AP

Synonyms

C5orf4, fatty acid hydroxylase domain containing 2, FAXDC2, Uncharacterized protein C5orf4

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using C5orf4 Polyclonal antibody (22046-1-AP)

    WB analysis of COLO 320 using 22046-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22046-1-AP (C5orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C5orf4 Polyclonal antibody (22046-1-AP)

    WB analysis of HepG2 using 22046-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22046-1-AP (C5orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using C5orf4 Polyclonal antibody (22046-1-AP)

    WB analysis of A549 using 22046-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22046-1-AP (C5orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using C5orf4 Polyclonal antibody (22046-1-AP)

    IHC staining of human liver using 22046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using C5orf4 Polyclonal antibody (22046-1-AP)

    IHC staining of human liver using 22046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using C5orf4 Polyclonal antibody (22046-1-AP)

    IHC staining of human kidney using 22046-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using C5orf4 Polyclonal antibody (22046-1-AP)

    IHC staining of human kidney using 22046-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inCOLO 320 cells, A549 cells, HepG2 cells
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 22046-1-AP is C5orf4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C5orf4 fusion protein Ag16960
Full Name chromosome 5 open reading frame 4
Calculated Molecular Weight 333 aa, 39 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC007216
Gene Symbol C5orf4
Gene ID (NCBI) 10826
RRIDAB_2878979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acid hydroxylase domain containing 2 (FAXDC2), also known as C5orf4, is a 40 kDa protein. The function of FAXDC2 remains largely unknown. The MW of this protein is 25 kDa, and this antibody specially recognises the 25 kDa protein.

Protocols

Product Specific Protocols
WB protocol for C5orf4 antibody 22046-1-APDownload protocol
IHC protocol for C5orf4 antibody 22046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Blood Cancer J

Novel function of FAXDC2 in megakaryopoiesis.

Authors - Q Jin
View Article
mouseWB

J Clin Invest

The cholesterol biosynthesis enzyme FAXDC2 couples Wnt/β-catenin to RTK/MAPK signaling

Authors - Babita Madan
View Article