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C5orf33 Polyclonal antibody

C5orf33 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26352-1-AP

Synonyms

C5orf33, NAD kinase 2, NADK2, UPF0465 protein C5orf33

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using C5orf33 Polyclonal antibody (26352-1-AP)

    WB analysis of mouse liver using 26352-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26352-1-AP (C5orf33 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using C5orf33 Polyclonal antibody (26352-1-AP)

    WB analysis of HL-60 using 26352-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26352-1-AP (C5orf33 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using C5orf33 Polyclonal antibody (26352-1-AP)

    IHC staining of human liver using 26352-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26352-1-AP (C5orf33 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using C5orf33 Polyclonal antibody (26352-1-AP)

    IHC staining of human liver using 26352-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26352-1-AP (C5orf33 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C5orf33 Polyclonal antibody (26352-1-AP)

    IF Staining of HeLa using 26352-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26352-1-AP (C5orf33 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, HL-60 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 26352-1-AP is C5orf33 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C5orf33 fusion protein Ag23706
Full Name chromosome 5 open reading frame 33
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC062567
Gene Symbol C5orf33
Gene ID (NCBI) 133686
RRIDAB_2880487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C5orf33 antibody 26352-1-APDownload protocol
IHC protocol for C5orf33 antibody 26352-1-APDownload protocol
IF protocol for C5orf33 antibody 26352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FASEB J

G-protein-coupled estrogen receptor 1 promotes peritoneal metastasis of gastric cancer through nicotinamide adenine dinucleotide kinase 1-mediated redox modulation

Authors - Teng Wu
View Article