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C5orf24 Polyclonal antibody

C5orf24 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 24319-1-AP

Synonyms

C5orf24, UPF0461 protein C5orf24

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using C5orf24 Polyclonal antibody (24319-1-AP)

    WB analysis of HEK-293 using 24319-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24319-1-AP (C5orf24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using C5orf24 Polyclonal antibody (24319-1-AP)

    IP experiment of HEK-293 using 24319-1-AP

    IP result of anti-C5orf24 (IP:24319-1-AP, 4ug; Detection:24319-1-AP 1:1000) with HEK-293 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using C5orf24 Polyclonal antibody (24319-1-AP)

    IHC staining of human pancreas using 24319-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using C5orf24 Polyclonal antibody (24319-1-AP)

    IHC staining of human pancreas using 24319-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using C5orf24 Polyclonal antibody (24319-1-AP)

    IHC staining of human kidney using 24319-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using C5orf24 Polyclonal antibody (24319-1-AP)

    IHC staining of human kidney using 24319-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C5orf24 Polyclonal antibody (24319-1-AP)

    IF Staining of HEK-293 using 24319-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C5orf24 antibody (24319-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using C5orf24 Polyclonal antibody (24319-1-AP)

    FC experiment of HEK-293 using 24319-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug C5orf24 Polyclonal antibody (24319-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24319-1-AP targets C5orf24 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitypig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C5orf24 fusion protein Ag19534
Full Name chromosome 5 open reading frame 24
Calculated Molecular Weight 188 aa, 20 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC053677
Gene Symbol C5orf24
Gene ID (NCBI) 134553
RRIDAB_2879491
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C5orf24 has two isoforms with MW 20 kDa and 16 kDa. For phosphoserine modification, the MW is about 20-23 kDa.

Protocols

Product Specific Protocols
WB protocol for C5orf24 antibody 24319-1-APDownload protocol
IHC protocol for C5orf24 antibody 24319-1-APDownload protocol
IF protocol for C5orf24 antibody 24319-1-APDownload protocol
IP protocol for C5orf24 antibody 24319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Front Immunol

Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.

Authors - Xu Ye
View Article