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C4orf49 Polyclonal antibody

C4orf49 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22569-1-AP

Synonyms

C4orf49, CESP-1, Corneal endothelium-specific protein 1, HUMMR, MGARP, OSAP, Ovary specific acidic protein, Protein MGARP

Validation Data Gallery

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  • Western Blot (WB) analysis of A2780 cells using C4orf49 Polyclonal antibody (22569-1-AP)

    WB analysis of A2780 using 22569-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using C4orf49 Polyclonal antibody (22569-1-AP)

    WB analysis of SKOV-3 using 22569-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using C4orf49 Polyclonal antibody (22569-1-AP)

    WB analysis of A549 using 22569-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using C4orf49 Polyclonal antibody (22569-1-AP)

    IHC staining of human ovary using 22569-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using C4orf49 Polyclonal antibody (22569-1-AP)

    IHC staining of human testis using 22569-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human ovary tissue using C4orf49 Polyclonal antibody (22569-1-AP)

    IHC staining of human ovary using 22569-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using C4orf49 Polyclonal antibody (22569-1-AP)

    IHC staining of human testis using 22569-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inA2780 cells, A549 cells, SKOV-3 cells
Positive IHC detected inhuman ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22569-1-AP is C4orf49 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C4orf49 fusion protein Ag16552
Full Name chromosome 4 open reading frame 49
Calculated Molecular Weight 240 aa, 25 kDa
Observed Molecular Weight 33-36 kDa
GenBank Accession NumberBC104174
Gene Symbol C4orf49
Gene ID (NCBI) 84709
RRIDAB_2879124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein MGARP, also named as C4ORF49, CESP1, HUMMR, OSAP and GS3582, plays a role in the trafficking of mitochondria along microtubules. It regulates the kinesin-mediated axonal transport of mitochondria to nerve terminals along microtubules during hypoxia. MGARP is localized to the mitochondria in cultured human CE, and its N-terminal seems to be processed after mitochondrial import. The MW of MGARP is about 33-36 kDa(PMID: 16565373).

Protocols

Product Specific Protocols
WB protocol for C4orf49 antibody 22569-1-APDownload protocol
IHC protocol for C4orf49 antibody 22569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols