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C3orf43 Polyclonal antibody

C3orf43 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25362-1-AP

Synonyms

C3orf43

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using C3orf43 Polyclonal antibody (25362-1-AP)

    WB analysis of mouse heart using 25362-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25362-1-AP (C3orf43 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using C3orf43 Polyclonal antibody (25362-1-AP)

    WB analysis of human plasma using 25362-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 25362-1-AP (C3orf43 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C3orf43 Polyclonal antibody (25362-1-AP)

    IF Staining of HepG2 using 25362-1-AP

    Immunofluorescent analysis of HepG2 cells using 25362-1-AP (C3orf43 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C3orf43 Polyclonal antibody (25362-1-AP)

    IF Staining of HeLa using 25362-1-AP

    Immunofluorescent analysis of HeLa cells using 25362-1-AP (C3orf43 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inmouse heart tissue, human plasma tissue
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25362-1-AP is C3orf43 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C3orf43 fusion protein Ag21670
Full Name chromosome 3 open reading frame 43
Calculated Molecular Weight 214 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC118636
Gene Symbol C3orf43
Gene ID (NCBI) 255798
RRIDAB_2880044
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C3orf43 antibody 25362-1-APDownload protocol
IF protocol for C3orf43 antibody 25362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols