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C3orf21 Polyclonal antibody

C3orf21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20913-1-AP

Synonyms

C3orf21, XXYLT1, xyloside xylosyltransferase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using C3orf21 Polyclonal antibody (20913-1-AP)

    WB analysis of HeLa using 20913-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20913-1-AP (C3orf21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using C3orf21 Polyclonal antibody (20913-1-AP)

    WB analysis of HeLa using 20913-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20913-1-AP (C3orf21 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using C3orf21 Polyclonal antibody (20913-1-AP)

    IHC staining of human prostate hyperplasia using 20913-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20913-1-AP (C3orf21 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using C3orf21 Polyclonal antibody (20913-1-AP)

    IHC staining of human prostate hyperplasia using 20913-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20913-1-AP (C3orf21 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C3orf21 Polyclonal antibody (20913-1-AP)

    IF Staining of HeLa using 20913-1-AP

    Immunofluorescent analysis of HeLa cells using 20913-1-AP (C3orf21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20913-1-AP is C3orf21 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C3orf21 fusion protein Ag15043
Full Name chromosome 3 open reading frame 21
Calculated Molecular Weight 393 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC039067
Gene Symbol C3orf21
Gene ID (NCBI) 152002
RRIDAB_11182820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C3orf21 antibody 20913-1-APDownload protocol
IHC protocol for C3orf21 antibody 20913-1-APDownload protocol
IF protocol for C3orf21 antibody 20913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Beta-Elemene Reduces the Malignancy of Non-Small Cell Lung Cancer by Enhancing C3orf21 Expression.

Authors - Hu Cai
View Article