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C3orf20 Polyclonal antibody

C3orf20 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20906-1-AP

Synonyms

C3orf20

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using C3orf20 Polyclonal antibody (20906-1-AP)

    WB analysis of human brain using 20906-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20906-1-AP (C3orf20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using C3orf20 Polyclonal antibody (20906-1-AP)

    WB analysis of mouse brain using 20906-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20906-1-AP (C3orf20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using C3orf20 Polyclonal antibody (20906-1-AP)

    IP experiment of mouse brain using 20906-1-AP

    IP result of anti-C3orf20 (IP:20906-1-AP, 3ug; Detection:20906-1-AP 1:500) with mouse brain tissue lysate 10000ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using C3orf20 Polyclonal antibody (20906-1-AP)

    IHC staining of human liver using 20906-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20906-1-AP (C3orf20 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using C3orf20 Polyclonal antibody (20906-1-AP)

    IHC staining of human liver using 20906-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20906-1-AP (C3orf20 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20906-1-AP is C3orf20 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C3orf20 fusion protein Ag15031
Full Name chromosome 3 open reading frame 20
Calculated Molecular Weight 904 aa, 101 kDa
Observed Molecular Weight 101 kDa, 88 kDa
GenBank Accession NumberBC038406
Gene Symbol C3orf20
Gene ID (NCBI) 84077
RRIDAB_10732599
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of C3orf20 has not been widely studied, and is yet to be fully elucidated. Two isoforms were produced by alternative splicing with predicted MW of 88 and 101 kDa according to UniProt. Catalog#20906-1-AP can recognize both 88 and 101 kDa isoforms.

Protocols

Product Specific Protocols
WB protocol for C3orf20 antibody 20906-1-APDownload protocol
IHC protocol for C3orf20 antibody 20906-1-APDownload protocol
IP protocol for C3orf20 antibody 20906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols