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C22orf33 Polyclonal antibody

C22orf33 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26577-1-AP

Synonyms

Testis-expressed protein 33, Protein EAN57, EAN57, CIMIP4, Ciliary microtubule inner protein 4

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using C22orf33 Polyclonal antibody (26577-1-AP)

    WB analysis of human testis using 26577-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 26577-1-AP (C22orf33 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using C22orf33 Polyclonal antibody (26577-1-AP)

    IF Staining of mouse testis using 26577-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using C22orf33 antibody (26577-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using C22orf33 Polyclonal antibody (26577-1-AP)

    IF Staining of U2OS using 26577-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 26577-1-AP (C22orf33 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman testis tissue
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26577-1-AP targets C22orf33 in WB, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C22orf33 fusion protein Ag23900
Full Name chromosome 22 open reading frame 33
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC042635
Gene Symbol C22orf33
Gene ID (NCBI) 339669
RRIDAB_2880558
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C22orf33 antibody 26577-1-APDownload protocol
IF protocol for C22orf33 antibody 26577-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols