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C21orf66 Polyclonal antibody

C21orf66 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21357-1-AP

Synonyms

PAX3- and PAX7-binding protein 1, GC-rich sequence DNA-binding factor 1, GCFC1, GCFC, fSAP105

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using C21orf66 Polyclonal antibody (21357-1-AP)

    WB analysis of human brain using 21357-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21357-1-AP (C21orf66 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using C21orf66 Polyclonal antibody (21357-1-AP)

    IHC staining of human pancreas cancer using 21357-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using C21orf66 Polyclonal antibody (21357-1-AP)

    IHC staining of human renal cell carcinoma using 21357-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using C21orf66 Polyclonal antibody (21357-1-AP)

    IHC staining of human renal cell carcinoma using 21357-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using C21orf66 Polyclonal antibody (21357-1-AP)

    IHC staining of mouse liver using 21357-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C21orf66 Polyclonal antibody (21357-1-AP)

    IF Staining of HeLa using 21357-1-AP

    Immunofluorescent analysis of Hela cells, using C21orf66 antibody 21357-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman renal cell carcinoma tissue, human pancreas cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

21357-1-AP targets C21orf66 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C21orf66 fusion protein Ag15789
Full Name chromosome 21 open reading frame 66
Calculated Molecular Weight 917 aa, 105 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberBC030539
Gene Symbol C21orf66
Gene ID (NCBI) 94104
RRIDAB_10733239
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C21orf66 antibody 21357-1-APDownload protocol
IHC protocol for C21orf66 antibody 21357-1-APDownload protocol
IF protocol for C21orf66 antibody 21357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Immunol

Paxbp1 is indispensable for the survival of CD4 and CD8 double-positive thymocytes

Authors - Wenting Li
View Article
mouseWB

Immunology

Paxbp1 is indispensable for the maintenance of peripheral CD4 T cell homeostasis

Authors - Wenting Li
View Article