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C21orf59 Polyclonal antibody

C21orf59 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21461-1-AP

Synonyms

Protein kurly homolog, C21orf48

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using C21orf59 Polyclonal antibody (21461-1-AP)

    WB analysis of K-562 using 21461-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 21461-1-AP (C21orf59 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using C21orf59 Polyclonal antibody (21461-1-AP)

    WB analysis of HeLa using 21461-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21461-1-AP (C21orf59 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using C21orf59 Polyclonal antibody (21461-1-AP)

    WB analysis of K-562 using 21461-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 21461-1-AP (C21orf59 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using C21orf59 Polyclonal antibody (21461-1-AP)

    WB analysis of PC-3 using 21461-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21461-1-AP (C21orf59 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using C21orf59 Polyclonal antibody (21461-1-AP)

    WB analysis of MCF-7 using 21461-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21461-1-AP (C21orf59 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C21orf59 Polyclonal antibody (21461-1-AP)

    IF Staining of HEK-293 using 21461-1-AP

    Immunofluorescent analysis of HEK-293 cells using 21461-1-AP (C21orf59 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, MCF-7 cells, PC-3 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21461-1-AP targets C21orf59 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C21orf59 fusion protein Ag14994
Full Name chromosome 21 open reading frame 59
Calculated Molecular Weight 290 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC000709
Gene Symbol C21orf59
Gene ID (NCBI) 56683
RRIDAB_2878863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C21orf59 antibody 21461-1-APDownload protocol
IF protocol for C21orf59 antibody 21461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways

Authors - Min Huang
View Article