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C20orf46 Polyclonal antibody

C20orf46 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 24678-1-AP

Synonyms

C20orf46, Transmembrane protein C20orf46

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using C20orf46 Polyclonal antibody (24678-1-AP)

    WB analysis of Jurkat using 24678-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24678-1-AP (C20orf46 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using C20orf46 Polyclonal antibody (24678-1-AP)

    WB analysis of mouse colon using 24678-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 24678-1-AP (C20orf46 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C20orf46 Polyclonal antibody (24678-1-AP)

    IHC staining of human colon cancer using 24678-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24678-1-AP (C20orf46 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C20orf46 Polyclonal antibody (24678-1-AP)

    IHC staining of human colon cancer using 24678-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24678-1-AP (C20orf46 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using C20orf46 Polyclonal antibody (24678-1-AP)

    IHC staining of human colon using 24678-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24678-1-AP (C20orf46 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using C20orf46 Polyclonal antibody (24678-1-AP)

    IHC staining of human colon using 24678-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24678-1-AP (C20orf46 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24678-1-AP is C20orf46 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C20orf46 fusion protein Ag20385
Full Name chromosome 20 open reading frame 46
Calculated Molecular Weight 256 aa, 28 kDa
GenBank Accession NumberBC156781
Gene Symbol C20orf46
Gene ID (NCBI) 55321
RRIDAB_2879669
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C20orf46, also named as TMEM74B, belongs to the TMEM74 family.

Protocols

Product Specific Protocols
WB protocol for C20orf46 antibody 24678-1-APDownload protocol
IHC protocol for C20orf46 antibody 24678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols