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  • Featured Product
  • KD/KO Validated

RTF2 Polyclonal antibody

RTF2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16633-1-AP

Synonyms

C20orf43, CDA05, CDAO5, HSPC164, HSPC169, RTF2, SHUJUN 3, UPF0549 protein C20orf43

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RTF2 Polyclonal antibody (16633-1-AP)

    WB analysis using 16633-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16633-1-AP (RTF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RTF2 Polyclonal antibody (16633-1-AP)

    WB analysis of HeLa using 16633-1-AP

    WB result of RTF2 antibody (16633-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RTF2 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using RTF2 Polyclonal antibody (16633-1-AP)

    IP experiment of HeLa using 16633-1-AP

    IP result of anti-RTF2 (IP:16633-1-AP, 3ug; Detection:16633-1-AP 1:500) with HeLa cells lysate 3440ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RTF2 Polyclonal antibody (16633-1-AP)

    IF Staining of HeLa using 16633-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RTF2 antibody (16633-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16633-1-AP targets RTF2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RTF2 fusion protein Ag9972
Full Name chromosome 20 open reading frame 43
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC003359
Gene Symbol RTF2
Gene ID (NCBI) 51507
RRIDAB_2256547
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of C20orf43 remains largely unknown. Catalog#16633-1-AP is a rabbit polyclonal antibody raised against the full-length of human C20orf43. The MW of this protein is 40 kDa, and this antibody specially recognises the 40 kDa protein.

Protocols

Product Specific Protocols
WB protocol for RTF2 antibody 16633-1-APDownload protocol
IF protocol for RTF2 antibody 16633-1-APDownload protocol
IP protocol for RTF2 antibody 16633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Removal of RTF2 from Stalled Replisomes Promotes Maintenance of Genome Integrity.

Authors - Molly C Kottemann
  • KD Validated
View Article
human,mouseWB

bioRxiv

RTF2 controls replication repriming and ribonucleotide excision at the replisome

Authors - Brooke A Conti
  • KD Validated
View Article