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C20orf4 Polyclonal antibody

C20orf4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20179-1-AP

Synonyms

C20orf4, CGI 23, PRO0225

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using C20orf4 Polyclonal antibody (20179-1-AP)

    WB analysis of MCF-7 using 20179-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20179-1-AP (C20orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using C20orf4 Polyclonal antibody (20179-1-AP)

    WB analysis of HeLa using 20179-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20179-1-AP (C20orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C20orf4 Polyclonal antibody (20179-1-AP)

    WB analysis of HepG2 using 20179-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20179-1-AP (C20orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20179-1-AP is C20orf4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C20orf4 fusion protein Ag14039
Full Name chromosome 20 open reading frame 4
Calculated Molecular Weight 384 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC001751
Gene Symbol C20orf4
Gene ID (NCBI) 25980
RRIDAB_2878653
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C20orf4 antibody 20179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols