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TEX38 Polyclonal antibody

TEX38 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24429-1-AP

Synonyms

C1orf223, Testis-expressed protein 38, ATPAF1-AS1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using TEX38 Polyclonal antibody (24429-1-AP)

    WB analysis of mouse testis using 24429-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24429-1-AP (TEX38 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using TEX38 Polyclonal antibody (24429-1-AP)

    WB analysis of MDA-MB-453s using 24429-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24429-1-AP (TEX38 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TEX38 Polyclonal antibody (24429-1-AP)

    IHC staining of human testis using 24429-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24429-1-AP (TEX38 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue, MDA-MB-453s cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

24429-1-AP targets TEX38 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TEX38 fusion protein Ag19833
Full Name chromosome 1 open reading frame 223
Calculated Molecular Weight 206 aa, 23 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC126318
Gene Symbol TEX38
Gene ID (NCBI) 374973
RRIDAB_2879544
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEX38, also named as ATPAF1-AS1 and C1orf223, is ATPAF1 antisense gene protein 1. This antibody detects 25-28 kDa with mouse testis and MDA-MB-453s cell lysate.

Protocols

Product Specific Protocols
WB protocol for TEX38 antibody 24429-1-APDownload protocol
IHC protocol for TEX38 antibody 24429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Adv Sci (Weinh)

Deciphering the Molecular Characteristics of Human Idiopathic Nonobstructive Azoospermia from the Perspective of Germ Cells

Authors - Yidong Chen
View Article