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  • Featured Product
  • KD/KO Validated

Bcl2 Polyclonal antibody

Bcl2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 26593-1-AP

Synonyms

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Bcl2 Polyclonal antibody (26593-1-AP)

    WB analysis using 26593-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Bcl2 Polyclonal antibody (26593-1-AP)

    WB analysis using 26593-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Bcl2 Polyclonal antibody (26593-1-AP)

    WB analysis using 26593-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Bcl2 Polyclonal antibody (26593-1-AP)

    WB analysis using 26593-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using Bcl2 Polyclonal antibody (26593-1-AP)

    WB analysis of mouse testis using 26593-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using Bcl2 Polyclonal antibody (26593-1-AP)

    WB analysis of mouse heart using 26593-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C2C12 cells using Bcl2 Polyclonal antibody (26593-1-AP)

    WB analysis of C2C12 using 26593-1-AP

    C2C12 cells were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Bcl2 Polyclonal antibody (26593-1-AP)

    IHC staining of mouse spleen using 26593-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Bcl2 Polyclonal antibody (26593-1-AP)

    IHC staining of mouse brain using 26593-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Bcl2 Polyclonal antibody (26593-1-AP)

    IHC staining of mouse brain using 26593-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Bcl2 Polyclonal antibody (26593-1-AP)

    IHC staining of mouse spleen using 26593-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Bcl2 Polyclonal antibody (26593-1-AP)

    IHC staining of mouse spleen using 26593-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Bcl2 Polyclonal antibody (26593-1-AP)

    IF Staining of NIH/3T3 using 26593-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Bcl2 antibody (26593-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Bcl2 Polyclonal antibody (26593-1-AP)

    IF Staining of NIH/3T3 using 26593-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (26593-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Bcl2 Polyclonal antibody (26593-1-AP)

    IF Staining of NIH/3T3 using 26593-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 26593-1-AP (Bcl2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inNIH/3T3 cells, C2C12 cells, mouse brain tissue, mouse heart tissue, mouse testis tissue, mouse kidney tissue, mouse spleen tissue, rat spleen tissue, rat kidney tissue, rat testis tissue, mouse colon tissue
Positive IHC detected inmouse spleen tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26593-1-AP targets Bcl2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Cited Reactivitymouse, rat, pig, canine, chicken, bovine, hamster, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bcl2 fusion protein Ag24260
Full Name B-cell leukemia/lymphoma 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberNM-009741
Gene Symbol Bcl2
Gene ID (NCBI) 12043
RRIDAB_2818996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1.       What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2.       FAQs for Bcl-2

  1.  How to measure Bcl-2 and Bax apoptotic markers by western blotting

    Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

  2. What band size should I expect in western blotting for Bcl-2?

    The molecular weight of Bcl-2 is 26 kDa.


Protocols

Product Specific Protocols
WB protocol for Bcl2 antibody 26593-1-APDownload protocol
IHC protocol for Bcl2 antibody 26593-1-APDownload protocol
IF protocol for Bcl2 antibody 26593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

ACS Nano

Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway

Authors - Xirui Xin
View Article
mouseWB

Acta Pharm Sin B

Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis

Authors - Yujun Zhou
View Article
mouseWB

Redox Biol

FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response

Authors - Zhe Wang
View Article
mouseWB

J Nanobiotechnology

PD-L1 blockade TAM-dependently potentiates mild photothermal therapy against triple-negative breast cancer

Authors - Chao Wang
View Article
mouseWB

Adv Healthc Mater

GDNF-Loaded Polydopamine Nanoparticles-Based Anisotropic Scaffolds Promote Spinal Cord Repair by Modulating Inhibitory Microenvironment

Authors - Jinjin Ma
View Article
WB

J Colloid Interface Sci

DNA Damage-Sensitized metal phenolic nanosynergists potentiate Low-Power phototherapy for osteosarcoma therapy

Authors - Chunxue Song
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Charlotte (Verified Customer) (08-15-2024)

The fainter band at the bottom of the membrane corresponds to the expected MW. Done in milk

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: NIH-3T3
Bcl2 Antibody Western Blot validation (1/1000 dilution) in NIH-3T3 (Cat no:26593-1-AP)
FH

Anna (Verified Customer) (10-04-2022)

Promising staining. I have a problem with attaching a photo file. Please check the file below. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 500
  • Cell Tissue Type: brain frozen sections - mouse
FH

PRADEEP (Verified Customer) (11-03-2020)

Giving very good result for IF.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse muscle