BRE Monoclonal antibody
BRE Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, rat, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2E5A5
Cat no : 66356-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | fetal human brain tissue, rat brain tissue |
Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66356-1-Ig targets BRE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | BRE fusion protein Ag2256 |
Full Name | brain and reproductive organ-expressed (TNFRSF1A modulator) |
Calculated Molecular Weight | 383 aa, 44 kDa |
Observed Molecular Weight | 42-47 kDa |
GenBank Accession Number | BC001251 |
Gene Symbol | BRE |
Gene ID (NCBI) | 9577 |
RRID | AB_2881735 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for BRE antibody 66356-1-Ig | Download protocol |
IHC protocol for BRE antibody 66356-1-Ig | Download protocol |
IF protocol for BRE antibody 66356-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |