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BPI Polyclonal antibody

BPI Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14368-1-AP

Synonyms

CAP57, CAP 57, Bactericidal permeability-increasing protein

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BPI Polyclonal antibody (14368-1-AP)

    IHC staining of human tonsillitis using 14368-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14368-1-AP (BPI antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BPI Polyclonal antibody (14368-1-AP)

    IF Staining of HepG2 using 14368-1-AP

    Immunofluorescent analysis of HepG2 cells, using BPI antibody 14368-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14368-1-AP targets BPI in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BPI fusion protein Ag5695
Full Name bactericidal/permeability-increasing protein
Calculated Molecular Weight 54 kDa
GenBank Accession NumberBC040955
Gene Symbol BPI
Gene ID (NCBI) 671
RRIDAB_2878047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for BPI antibody 14368-1-APDownload protocol
IF protocol for BPI antibody 14368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols