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BIVM Polyclonal antibody

BIVM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25432-1-AP

Synonyms

BIVM

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using BIVM Polyclonal antibody (25432-1-AP)

    WB analysis of Raji using 25432-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 25432-1-AP (BIVM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using BIVM Polyclonal antibody (25432-1-AP)

    IHC staining of human ovary using 25432-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25432-1-AP (BIVM Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using BIVM Polyclonal antibody (25432-1-AP)

    IHC staining of human ovary using 25432-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25432-1-AP (BIVM Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inRaji cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25432-1-AP is BIVM Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BIVM fusion protein Ag22087
Full Name basic, immunoglobulin-like variable motif containing
Calculated Molecular Weight 503 aa, 57 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC075084
Gene Symbol BIVM
Gene ID (NCBI) 54841
RRIDAB_2880076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BIVM has been identified using an electronic search based on the conservation of short sequence motifs within the variable region of immunoglobulin (Ig) genes. It is tightly linked (41 bp) and in the opposite transcriptional orientation to MGC5302 (also known as KDEL1 and EP58) in human. BIVM has two isoforms with MW 57 kDa and 32 kDa.

Protocols

Product Specific Protocols
WB protocol for BIVM antibody 25432-1-APDownload protocol
IHC protocol for BIVM antibody 25432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols