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  • Featured Product
  • KD/KO Validated

cIAP1 Polyclonal antibody

cIAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 10022-1-AP

Synonyms

API1, BIRC2, MIHB, RNF48, C-IAP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse skeletal muscle tissue using cIAP1 Polyclonal antibody (10022-1-AP)

    WB analysis of mouse skeletal muscle using 10022-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using cIAP1 Polyclonal antibody (10022-1-AP)

    WB analysis of mouse testis using 10022-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using cIAP1 Polyclonal antibody (10022-1-AP)

    WB analysis of mouse liver using 10022-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using cIAP1 Polyclonal antibody (10022-1-AP)

    WB analysis of mouse brain using 10022-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using cIAP1 Polyclonal antibody (10022-1-AP)

    WB analysis of Jurkat using 10022-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using cIAP1 Polyclonal antibody (10022-1-AP)

    IHC staining of human testis using 10022-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10022-1-AP (cIAP1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using cIAP1 Polyclonal antibody (10022-1-AP)

    IHC staining of human testis using 10022-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10022-1-AP (cIAP1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using cIAP1 Polyclonal antibody (10022-1-AP)

    IHC staining of mouse pancreas using 10022-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10022-1-AP (cIAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using cIAP1 Polyclonal antibody (10022-1-AP)

    IHC staining of mouse testis using 10022-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10022-1-AP (cIAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using cIAP1 Polyclonal antibody (10022-1-AP)

    IHC staining of human pancreas using 10022-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10022-1-AP (cIAP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using cIAP1 Polyclonal antibody (10022-1-AP)

    IHC staining of human pancreas using 10022-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10022-1-AP (cIAP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using cIAP1 Polyclonal antibody (10022-1-AP)

    IF Staining of HepG2 using 10022-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using cIAP1 antibody (10022-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using cIAP1 Polyclonal antibody (10022-1-AP)

    IF Staining of HepG2 using 10022-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10022-1-AP (cIAP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using cIAP1 Polyclonal antibody (10022-1-AP)

    FC experiment of HepG2 using 10022-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug cIAP1 Polyclonal antibody (10022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, mouse testis tissue
Positive IHC detected inhuman testis tissue, mouse pancreas tissue, mouse testis tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10022-1-AP targets cIAP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen cIAP1 fusion protein Ag15203
Full Name baculoviral IAP repeat-containing 2
Calculated Molecular Weight 618 aa, 70 kDa
Observed Molecular Weight 70 kDa, 55-60 kDa
GenBank Accession NumberBC016174
Gene Symbol cIAP1
Gene ID (NCBI) 329
RRIDAB_2063790
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460). This BIRC2 antibody (10022-1-AP) can bind both full length (70kd) and cleaved form (60kd) of the protein.

Protocols

Product Specific Protocols
WB protocol for cIAP1 antibody 10022-1-APDownload protocol
IHC protocol for cIAP1 antibody 10022-1-APDownload protocol
IF protocol for cIAP1 antibody 10022-1-APDownload protocol
FC protocol for cIAP1 antibody 10022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB,IF

Nat Genet

Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis.

Authors - Santoro Massimo M MM
  • KD Validated
View Article
humanIF

Mol Cell

Liquid phase separation of NEMO induced by polyubiquitin chains activates NF-κB.

Authors - Mingjian Du
View Article
humanWB

Mol Cell

A cytoplasmic ATM-TRAF6-cIAP1 module links nuclear DNA damage signaling to ubiquitin-mediated NF-κB activation.

Authors - Michael Hinz
  • KD Validated
View Article
mouseWB

Acta Pharm Sin B

A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.

Authors - Ziqiao Yuan
View Article
humanWB

Dev Cell

Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.

Authors - Maria Zachari
View Article
mouseWB

J Immunol

Differential TRAF3 utilization by a variant human CD40 receptor with enhanced signaling.

Authors - Peters Anna L AL
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

S (Verified Customer) (09-23-2024)

Excellent

  • Applications: Immunoprecipitation
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: A549
cIAP1 Antibody Immunoprecipitation validation (1000 dilution) in A549 (Cat no:10022-1-AP)