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  • Featured Product
  • KD/KO Validated

BIN2 Polyclonal antibody

BIN2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14245-1-AP

Synonyms

BIN2, BRAP 1, BRAP1, bridging integrator 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BIN2 Polyclonal antibody (14245-1-AP)

    WB analysis using 14245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using BIN2 Polyclonal antibody (14245-1-AP)

    WB analysis of U-937 using 14245-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using BIN2 Polyclonal antibody (14245-1-AP)

    WB analysis of Jurkat using 14245-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using BIN2 Polyclonal antibody (14245-1-AP)

    IP experiment of Jurkat using 14245-1-AP

    IP result of anti-BIN2 (IP:14245-1-AP, 4ug; Detection:14245-1-AP 1:400) with Jurkat cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human spleen tissue using BIN2 Polyclonal antibody (14245-1-AP)

    IHC staining of human spleen using 14245-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using BIN2 Polyclonal antibody (14245-1-AP)

    IHC staining of human spleen using 14245-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using BIN2 Polyclonal antibody (14245-1-AP)

    IHC staining of human lung using 14245-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using BIN2 Polyclonal antibody (14245-1-AP)

    IHC staining of human lung using 14245-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using BIN2 Polyclonal antibody (14245-1-AP)

    IHC staining of human heart using 14245-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using BIN2 Polyclonal antibody (14245-1-AP)

    IHC staining of human heart using 14245-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using BIN2 Polyclonal antibody (14245-1-AP)

    IHC staining of human skin using 14245-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using BIN2 Polyclonal antibody (14245-1-AP)

    IHC staining of human skin using 14245-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using BIN2 Polyclonal antibody (14245-1-AP)

    IHC staining of mouse heart using 14245-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14245-1-AP (BIN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using BIN2 Polyclonal antibody (14245-1-AP)

    IHC staining of mouse heart using 14245-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14245-1-AP (BIN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using BIN2 Polyclonal antibody (14245-1-AP)

    IHC staining of human ovary using 14245-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using BIN2 Polyclonal antibody (14245-1-AP)

    IHC staining of human ovary using 14245-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, U-937 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman spleen tissue, mouse heart tissue, human heart tissue, human skin tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14245-1-AP targets BIN2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BIN2 fusion protein Ag5502
Full Name bridging integrator 2
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC047686
Gene Symbol BIN2
Gene ID (NCBI) 51411
RRIDAB_2063647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846)

Protocols

Product Specific Protocols
WB protocol for BIN2 antibody 14245-1-APDownload protocol
IHC protocol for BIN2 antibody 14245-1-APDownload protocol
IP protocol for BIN2 antibody 14245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

J Clin Invest

BIN2 orchestrates platelet calcium signaling in thrombosis and thrombo-inflammation.

Authors - Julia Volz
  • KO Validated
View Article