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BHMT2 Polyclonal antibody

BHMT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16351-1-AP

Synonyms

BHMT2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using BHMT2 Polyclonal antibody (16351-1-AP)

    WB analysis of mouse liver using 16351-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16351-1-AP (BHMT2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using BHMT2 Polyclonal antibody (16351-1-AP)

    WB analysis of rat liver using 16351-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 16351-1-AP (BHMT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using BHMT2 Polyclonal antibody (16351-1-AP)

    IHC staining of human kidney using 16351-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16351-1-AP (BHMT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using BHMT2 Polyclonal antibody (16351-1-AP)

    IHC staining of human kidney using 16351-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16351-1-AP (BHMT2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16351-1-AP targets BHMT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BHMT2 fusion protein Ag9558
Full Name betaine-homocysteine methyltransferase 2
Calculated Molecular Weight 363 aa, 40 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC020665
Gene Symbol BHMT2
Gene ID (NCBI) 23743
RRIDAB_2878248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BHMT2 antibody 16351-1-APDownload protocol
IHC protocol for BHMT2 antibody 16351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols