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BCAS3 Polyclonal antibody

BCAS3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10402-1-AP

Synonyms

BCAS3, GAOB1, MAAB, Protein Maab1, Rudhira

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCAS3 Polyclonal antibody (10402-1-AP)

    WB analysis using 10402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10402-1-AP (BCAS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using BCAS3 Polyclonal antibody (10402-1-AP)

    WB analysis of A431 using 10402-1-AP

    WB result of BCAS3 antibody (10402-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAS3 transfected A431 cells.

Tested Applications

Positive WB detected inA549 cells, A431 cells, HeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10402-1-AP targets BCAS3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCAS3 fusion protein Ag0646
Full Name breast carcinoma amplified sequence 3
Calculated Molecular Weight 99 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC001250
Gene Symbol BCAS3
Gene ID (NCBI) 54828
RRIDAB_10897181
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rudhira/BCAS3 (Breast Cancer Amplified Sequence 3) is a conserved protein expressed in the embryonic vasculature and malignant tumors. Rudhira is a predicted WD40 domain protein. During mouse development Rudhira is expressed in erythropoietic and angiogenic precursors but not in their differentiated progeny. This transient expression of Rudhira is also seen in normal adult angiogenesis. BCAS3, the human ortholog of Rudhira is 98% identical and the gene maps to a breakpoint of hematological neoplasms on chromosome 17q23.1. BCAS3 is also expressed in vascular development in embryoid bodies in vitro and was originally identified as a gene over-expressed in breast cancers. It is reported to be a target of metastasis associated protein-1 (MTA-1). Further BCAS3 expression is also upregulated in grade III and IV glioblastomas where it is misexpressed in the tumor cells.

Protocols

Product Specific Protocols
WB protocol for BCAS3 antibody 10402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols